Membrane Anchored Polymers Modulate Amyloid Fibrillation

The nucleating role of cellular membrane components, such as lipid moieties on amyloid beta (A?1–40) fibrillation, has been reported in recent years. influence conjugates fabricated from anchors (cholesterol, diacylglycerol) and hydrophilic polymers A?1–40 fibrillation is here, aiming to understand the impact cloud point temperature (Tcp) its hydrophobic tails fibrillation. Novel lipid-polymer conjugates, consisting poly(oligo(ethylene glycol)macrylates) groups (diacylglyceryl-, cholesteryl-, octyl-, decyl-, hexadecyl-) are synthesized using reversible addition-fragmentation chain transfer (RAFT) polymerization, allowing tune hydrophilic-hydrophobic profile by varying both, degree polymerization (n) number ethylene glycol units (m) their side chain. these investigated via vitro kinetic studies transmission electron microscopy (TEM). Hydrophobic lipid-anchors significantly delaying (both lag- half times), observing similar fibrillar structures TEM when compared native A?1–40. Other end also A?1–40, irrespective “n” “m,” whereas more with longer glycol-sidechains, m = 3 for octyl, decyl 5 cholesterol) only marginally inhibited